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The objective of this work is the extraction by coacervate of Eosin B. Its extraction has been achieved in aqueous solution by using the extractant triton X-100 (iso-octyl phenoxy polyethoxy ethanol) and dehydol Lt7 which are easily and rapidly biodegradable. The experimental results of the extraction, as a function of the concentration of the surfactant and the temperature, are expressed by the following three quantities: percentage of dye extracted (E), residual concentration of dye in the phase dilute (Xs, w) and volume fraction of the coacervate (Φc) at equilibrium. The results obtained for each parameter are represented on three-dimensional diagrams by using the empirical smoothing method. The calculated values are consistent with the measurements. For OXoC15E7, the extraction yields achieved under optimal conditions with TX-100 surfactant are 99.18% and 98% successively. The results revealed that the reduction of the solute concentration in the dilute phase is about 48 and 88.5 times with Dehydol LT7 and TX-100, respectively. Besides, the addition of sodium sulfate is also investigated.